ABSTRACT
Objective To identify the single nucleotide polymorphism (SNP) of the gene of TNIP1 using high resolution melting (HRM) analysis with unlabeled probe,and funther analyse the association with systemic lupus erythematosuscsle.Methods 297 patients that fulfilled the American College of Rheumatology criteria for SLE and 351 ethnically matched healthy controls were recruited from Shenzhen Hospital of Peking University.The association of TNIP1 SNP rs7708392 (G/C) was determined by high resolution melting (HRM) analysis with unlabeled probe in SLE patients and healthy controls.Results HRMA with unlabeled probe successfully distinguished all genotypes.Genotype frequencies of GG,GC and CC among SLE patients were 36.0％,51.5％ and 12.5％,respectively,while the frequencies among healthy control were 32.5％,46.7％ and 20.8％.Statistically significant differences were observed in both genotype frequencies for rs7708392 in the SLE patients as compared with the controls.Minor allele (C) of rs7708392 (P=0.031,OR 0.78,95％ CI 0.63～0.98) was found to be protective against SLE.The association of SNP rs7708392 with the diagnostic criteria of SLE was also examined.Minor allele (C) exerts protective effect on the incidence of arthritis (P=0.013,OR=0.65,95 ％ CI=0.47 ～ 0.92) and abnormalities of antinuclear antibody (P =0.022,OR =0.68,95 ％ CI =0.49 ～ 0.95).TNIP1 SNPs were irrelevant to other diagnostic criteria of SLE.Conclusion Polymorphisms of rs7708392 in TNIP1 gene were associated with disease risk,as well as arthritis and autoantibody production,of systemic lupus erythematosus in Chinese population.